High-frequency cotransformation by copolymerization of plasmids in the fission yeast Schizosaccharomyces pombe.

We have developed a high-frequency cotransformation system which is useful in introducing nonreplicating circular DNA plasmids into the fission yeast Schizosaccharomyces pombe. This system depends on two factors: the ability of the ural-complementing helper plasmids pFYM2 and pFYM225 to propagate au...

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izdano v:	Mol Cell Biol
Principais autores:	Sakai, K, Sakaguchi, J, Yamamoto, M
Format:	Artigo
Jezik:	Inglês
Izdano:	Taylor & Francis 1984
Teme:	Research Article
Online dostop:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC368774/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6717440/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.4.4.651-656.1984
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