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Human Glx2 contains an Fe(II)Zn(II) center but is active as a mononuclear Zn(II) enzyme()

Human glyoxalase II (Glx2) was over-expressed in rich medium and in minimal medium containing zinc, iron, or cobalt, and the resulting Glx2 analogs were characterized using metal analyses, steady-state and pre-steady state kinetics, and NMR and EPR spectroscopies in order to determine the nature of...

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Detalhes bibliográficos
Publicado no:Biochemistry
Main Authors: Limphong, Pattraranee, McKinney, Ross M., Adams, Nicole E., Bennett, Brian, Makaroff, Christopher A., Gunasekera, Thusitha, Crowder, Michael W.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4627686/
https://ncbi.nlm.nih.gov/pubmed/19413286
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi9001375
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