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An improved Tn7-lux reporter for broad host range, chromosomally-integrated promoter fusions in Gram-negative bacteria

An improved vector for chromosomally-integrated promoter-lux fusions is described. The modified vector was tested in parallel with the unmodified vector using the well-characterized E. coli araBAD promoter in the Pseudomonas aeruginosa attTn7 site. The modified mini-Tn7 showed reduced background lum...

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Detalhes bibliográficos
Publicado no:J Microbiol Methods
Main Authors: Glassing, Angela, Lewis, Thomas A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4624500/
https://ncbi.nlm.nih.gov/pubmed/26341612
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2015.08.016
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