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Broad-Host-Range Expression Reveals Native and Host Regulatory Elements That Influence Heterologous Antibiotic Production in Gram-Negative Bacteria

Heterologous expression has become a powerful tool for studying microbial biosynthetic gene clusters (BGCs). Here, we extend the transformation-associated recombination cloning and heterologous expression platform for microbial BGCs to include Gram-negative proteobacterial expression hosts. Using a...

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Detalhes bibliográficos
Publicado no:mBio
Main Authors: Zhang, Jia Jia, Tang, Xiaoyu, Zhang, Michelle, Nguyen, Darlene, Moore, Bradley S.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5587914/
https://ncbi.nlm.nih.gov/pubmed/28874475
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mBio.01291-17
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