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Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.

A broad host range cloning vehicle that can be mobilized at high frequency into Gram-negative bacteria has been constructed from the naturally occurring antibiotic resistance plasmid RK2. The vehicle is 20 kilobase pairs in size, encodes tetracycline resistance, and contains two single restriction e...

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Detalhes bibliográficos
Principais autores: Ditta, G, Stanfield, S, Corbin, D, Helinski, D R
Formato: Artigo
Idioma:Inglês
Publicado em: 1980
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC350500/
https://ncbi.nlm.nih.gov/pubmed/7012838
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