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Construction of Mobilizable Mini-Tn7 Vectors for Bioluminescent Detection of Gram-Negative Bacteria and Single-Copy Promoter lux Reporter Analysis
We describe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3697578/ https://ncbi.nlm.nih.gov/pubmed/23584769 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00640-13 |
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