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Preparation and identification of anti-transforming growth factor β1 U1 small nuclear RNA chimeric ribozyme in vitro

AIM: To study the preparation and cleavage activity of anti-transforming growth factor (TGF)β1 U1 small nuclear (sn) RNA chimeric hammerhead ribozymes in vitro. METHODS: TGFβ1 partial gene fragment was cloned into T-vector at the downstream of T7 promoter. (32)p-labeled TGFβ1 partial transcripts as...

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Detalhes bibliográficos
Publicado no:World J Gastroenterol
Main Authors: Lin, Ju-Sheng, Song, Yu-Hu, Kong, Xin-Juan, Li, Bin, Liu, Nan-Zhi, Wu, Xiao-Li, Jin, You-Xin
Formato: Artigo
Idioma:Inglês
Publicado em: Baishideng Publishing Group Inc 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4621585/
https://ncbi.nlm.nih.gov/pubmed/12632521
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v9.i3.572
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