Preparation and identification of anti-transforming growth factor β1 U1 small nuclear RNA chimeric ribozyme in vitro

AIM: To study the preparation and cleavage activity of anti-transforming growth factor (TGF)β1 U1 small nuclear (sn) RNA chimeric hammerhead ribozymes in vitro. METHODS: TGFβ1 partial gene fragment was cloned into T-vector at the downstream of T7 promoter. (32)p-labeled TGFβ1 partial transcripts as...

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Vydáno v:World J Gastroenterol
Hlavní autoři: Lin, Ju-Sheng, Song, Yu-Hu, Kong, Xin-Juan, Li, Bin, Liu, Nan-Zhi, Wu, Xiao-Li, Jin, You-Xin
Médium: Artigo
Jazyk:Inglês
Vydáno: Baishideng Publishing Group Inc 2003
Témata:
Basic Research
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4621585/
https://ncbi.nlm.nih.gov/pubmed/12632521
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v9.i3.572
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