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PCR-Free Enrichment of Mitochondrial DNA from Human Blood and Cell Lines for High Quality Next-Generation DNA Sequencing

Recent advances in sequencing technology allow for accurate detection of mitochondrial sequence variants, even those in low abundance at heteroplasmic sites. Considerable sequencing cost savings can be achieved by enriching samples for mitochondrial (relative to nuclear) DNA. Reduction in nuclear DN...

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Bibliographische Detailangaben
Veröffentlicht in:PLoS One
Hauptverfasser: Gould, Meetha P., Bosworth, Colleen M., McMahon, Sarah, Grandhi, Sneha, Grimerg, Brian T., LaFramboise, Thomas
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2015
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4619561/
https://ncbi.nlm.nih.gov/pubmed/26488301
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0139253
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