DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs

BACKGROUND: CRISPR genome-editing technology makes it possible to quickly and cheaply delete non-protein-coding regulatory elements. We present a vector system adapted for this purpose called DECKO (Double Excision CRISPR Knockout), which applies a simple two-step cloning to generate lentiviral vect...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:BMC Genomics
Hlavní autoři: Aparicio-Prat, Estel, Arnan, Carme, Sala, Ilaria, Bosch, Núria, Guigó, Roderic, Johnson, Rory
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2015
Témata:
Methodology Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4619085/
https://ncbi.nlm.nih.gov/pubmed/26493208
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-015-2086-z
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo otaguje tento záznam!

Podobné jednotky