Cloning of HBsAg-encoded genes in different vectors and their expression in eukaryotic cells

AIM: To compare the efficiency of different plasmids as DNA vectors by cloning three HBsAg-encoded genes into two eukaryotic expression vectors, pRc/CMV and pSG5UTPL/Flag, and to express HBsAg S, MS, and LS proteins in SP2/0 cells, and to establish monoclone SP2/0 cell strains that are capable of ex...

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Vydáno v:World J Gastroenterol
Hlavní autoři: Qin, Shan, Tang, Hong, Zhao, Lian-San, He, Fang, Lin, Yong, Liu, Li, He, Xiao-Mei
Médium: Artigo
Jazyk:Inglês
Vydáno: Baishideng Publishing Group Inc 2003
Témata:
Brief Reports
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4611384/
https://ncbi.nlm.nih.gov/pubmed/12717868
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v9.i5.1111
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