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Cloning of HBsAg-encoded genes in different vectors and their expression in eukaryotic cells
AIM: To compare the efficiency of different plasmids as DNA vectors by cloning three HBsAg-encoded genes into two eukaryotic expression vectors, pRc/CMV and pSG5UTPL/Flag, and to express HBsAg S, MS, and LS proteins in SP2/0 cells, and to establish monoclone SP2/0 cell strains that are capable of ex...
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| Publicado no: | World J Gastroenterol |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Baishideng Publishing Group Inc
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4611384/ https://ncbi.nlm.nih.gov/pubmed/12717868 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v9.i5.1111 |
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