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Isolation of Endoplasmic Reticulum, Mitochondria, and Mitochondria-Associated Membrane and Detergent Resistant Membrane Fractions from Transfected Cells and from Human Cytomegalovirus-Infected Primary Fibroblasts

Increasingly mechanistic virology studies require dependable and sensitive methods for isolating purified organelles containing functional cellular sub-domains. The mitochondrial network is, in part, closely apposed to the endoplasmic reticulum (ER). The mitochondria-associated membrane (MAM) fracti...

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Detalhes bibliográficos
Publicado no:Curr Protoc Cell Biol
Main Authors: Williamson, Chad D., Wong, Daniel S., Bozidis, Petros, Zhang, Aiping, Colberg-Poley, Anamaris M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4607254/
https://ncbi.nlm.nih.gov/pubmed/26331984
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471143030.cb0327s68
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