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Three-dimensional super-resolution protein localization correlated with vitrified cellular context

We demonstrate the use of cryogenic super-resolution correlative light and electron microscopy (csCLEM) to precisely determine the spatial relationship between proteins and their native cellular structures. Several fluorescent proteins (FPs) were found to be photoswitchable and emitted far more phot...

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Salvato in:
Dettagli Bibliografici
Pubblicato in:Sci Rep
Autori principali: Liu, Bei, Xue, Yanhong, Zhao, Wei, Chen, Yan, Fan, Chunyan, Gu, Lusheng, Zhang, Yongdeng, Zhang, Xiang, Sun, Lei, Huang, Xiaojun, Ding, Wei, Sun, Fei, Ji, Wei, Xu, Tao
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group 2015
Soggetti:
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4604464/
https://ncbi.nlm.nih.gov/pubmed/26462878
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep13017
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