Three-dimensional super-resolution protein localization correlated with vitrified cellular context

We demonstrate the use of cryogenic super-resolution correlative light and electron microscopy (csCLEM) to precisely determine the spatial relationship between proteins and their native cellular structures. Several fluorescent proteins (FPs) were found to be photoswitchable and emitted far more phot...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Liu, Bei, Xue, Yanhong, Zhao, Wei, Chen, Yan, Fan, Chunyan, Gu, Lusheng, Zhang, Yongdeng, Zhang, Xiang, Sun, Lei, Huang, Xiaojun, Ding, Wei, Sun, Fei, Ji, Wei, Xu, Tao
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2015
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4604464/
https://ncbi.nlm.nih.gov/pubmed/26462878
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep13017
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