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Three-dimensional super-resolution protein localization correlated with vitrified cellular context

We demonstrate the use of cryogenic super-resolution correlative light and electron microscopy (csCLEM) to precisely determine the spatial relationship between proteins and their native cellular structures. Several fluorescent proteins (FPs) were found to be photoswitchable and emitted far more phot...

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Библиографические подробности
Опубликовано в: :Sci Rep
Главные авторы: Liu, Bei, Xue, Yanhong, Zhao, Wei, Chen, Yan, Fan, Chunyan, Gu, Lusheng, Zhang, Yongdeng, Zhang, Xiang, Sun, Lei, Huang, Xiaojun, Ding, Wei, Sun, Fei, Ji, Wei, Xu, Tao
Формат: Artigo
Язык:Inglês
Опубликовано: Nature Publishing Group 2015
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4604464/
https://ncbi.nlm.nih.gov/pubmed/26462878
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep13017
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