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Three-dimensional super-resolution protein localization correlated with vitrified cellular context
We demonstrate the use of cryogenic super-resolution correlative light and electron microscopy (csCLEM) to precisely determine the spatial relationship between proteins and their native cellular structures. Several fluorescent proteins (FPs) were found to be photoswitchable and emitted far more phot...
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| Опубликовано в: : | Sci Rep |
|---|---|
| Главные авторы: | , , , , , , , , , , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Nature Publishing Group
2015
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4604464/ https://ncbi.nlm.nih.gov/pubmed/26462878 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep13017 |
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