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Protein Quantification Using Controlled DNA Melting Transitions in Bivalent Probe Assemblies

Homogenous protein assays, despite the potential for mix-and-read workflows, have eluded widespread acceptance due to interferences in biological matrices and limited multiplexability. Here, we employ standard qPCR instrumentation for thermofluorimetric analysis of bivalent probe (TFAB) assemblies,...

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Bibliografske podrobnosti
izdano v:Anal Chem
Main Authors: Kim, Joonyul, Hu, Juan, Bezerra, Andresa B., Holtan, Mark D., Brooks, Jessica C., Easley, Christopher J.
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4601099/
https://ncbi.nlm.nih.gov/pubmed/26372070
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.5b03432
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