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Protein Quantification Using Controlled DNA Melting Transitions in Bivalent Probe Assemblies
Homogenous protein assays, despite the potential for mix-and-read workflows, have eluded widespread acceptance due to interferences in biological matrices and limited multiplexability. Here, we employ standard qPCR instrumentation for thermofluorimetric analysis of bivalent probe (TFAB) assemblies,...
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| Publicado no: | Anal Chem |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4601099/ https://ncbi.nlm.nih.gov/pubmed/26372070 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.5b03432 |
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