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Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells

Luminescence Resonance Energy Transfer, or LRET, is a powerful technique used to measure distances between two sites in proteins within the distance range of 10-100 Å. By measuring the distances under various ligated conditions, conformational changes of the protein can be easily assessed. With LRET...

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Bibliographische Detailangaben
Veröffentlicht in:J Vis Exp
Hauptverfasser: Dolino, Drew M., Ramaswamy, Swarna S., Jayaraman, Vasanthi
Format: Artigo
Sprache:Inglês
Veröffentlicht: MyJove Corporation 2014
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4596616/
https://ncbi.nlm.nih.gov/pubmed/25285993
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51895
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