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Probing the Chemical Mechanism of Saccharopine Reductase from Saccharomyces cerevisiae Using Site-Directed Mutagenesis
Saccharopine reductase catalyzes the reductive amination of L-α-aminoadipate-δ-semialdehyde with L-glutamate to give saccharopine. Two mechanisms have been proposed for the reductase, one that makes use of enzyme side chains as acid-base catalytic groups, and a second, in which the reaction is catal...
Gardado en:
| Publicado en: | Arch Biochem Biophys |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2015
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4596410/ https://ncbi.nlm.nih.gov/pubmed/26342457 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2015.08.023 |
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