Super-Resolution Imaging of the Golgi in Live Cells with a Bio-orthogonal Ceramide Probe()

We report a lipid-based strategy to visualize Golgi structure and dynamics at super-resolution in live cells. The method is based on two novel reagents: a trans-cyclooctene-containing ceramide lipid (Cer-TCO) and a highly reactive, tetrazine-tagged near-IR dye (SiR-Tz). These reagents assemble via a...

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Pubblicato in:Angew Chem Int Ed Engl
Autori principali: Erdmann, Roman S., Takakura, Hideo, Thompson, Alexander D., Rivera-Molina, Felix, Allgeyer, Edward S., Bewersdorf, Joerg, Toomre, Derek K., Schepartz, Alanna
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2014
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4593319/
https://ncbi.nlm.nih.gov/pubmed/25081303
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201403349
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