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STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells

Long time-lapse super-resolution imaging in live cells requires a labeling strategy that combines a bright, photostable fluorophore with a high-density localization probe. Lipids are ideal high-density localization probes, as they are >100 times more abundant than most membrane-bound proteins and...

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Vydáno v:Methods Mol Biol
Hlavní autoři: Erdmann, Roman S., Toomre, Derek, Schepartz, Alanna
Médium: Artigo
Jazyk:Inglês
Vydáno: 2017
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6146391/
https://ncbi.nlm.nih.gov/pubmed/28924659
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7265-4_6
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