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Improved OTU-picking using long-read 16S rRNA gene amplicon sequencing and generic hierarchical clustering

BACKGROUND: High-throughput bacterial 16S rRNA gene sequencing followed by clustering of short sequences into operational taxonomic units (OTUs) is widely used for microbiome profiling. However, clustering of short 16S rRNA gene reads into biologically meaningful OTUs is challenging, in part because...

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Detalhes bibliográficos
Publicado no:Microbiome
Main Authors: Franzén, Oscar, Hu, Jianzhong, Bao, Xiuliang, Itzkowitz, Steven H., Peter, Inga, Bashir, Ali
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4593230/
https://ncbi.nlm.nih.gov/pubmed/26434730
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s40168-015-0105-6
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