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Development of a Tightly Controlled Off Switch for Saccharomyces cerevisiae Regulated by Camphor, a Low-Cost Natural Product

Here we describe the engineering of a distant homolog of the Tet repressor, CamR, isolated from Pseudomonas putida, that is regulated by camphor, a very inexpensive small molecule (at micromolar concentrations) for use in Saccharomyces cerevisiae. The repressor was engineered by expression from a co...

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Détails bibliographiques
Publié dans:G3 (Bethesda)
Auteurs principaux: Ikushima, Shigehito, Zhao, Yu, Boeke, Jef D.
Format: Artigo
Langue:Inglês
Publié: Genetics Society of America 2015
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4592980/
https://ncbi.nlm.nih.gov/pubmed/26206350
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.114.012765
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