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Superloser: A Plasmid Shuffling Vector for Saccharomyces cerevisiae with Exceedingly Low Background

Here we report a new plasmid shuffle vector for forcing budding yeast (Saccharomyces cerevisiae) to incorporate a new genetic pathway in place of a native pathway – even an essential one – while maintaining low false positive rates (less than 1 in 10(8) per cell). This plasmid, dubbed “Superloser,”...

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Detalles Bibliográficos
Publicado en:G3 (Bethesda)
Main Authors: Haase, Max A. B., Truong, David M., Boeke, Jef D.
Formato: Artigo
Idioma:Inglês
Publicado: Genetics Society of America 2019
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6686923/
https://ncbi.nlm.nih.gov/pubmed/31213518
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.119.400325
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