Continuous directed evolution of DNA-binding proteins to improve TALEN specificity

Nucleases containing programmable DNA-binding domains can alter the genomes of model organisms and have the potential to become human therapeutics. Here we present DNA-binding phage-assisted continuous evolution (DB-PACE) as a general approach for the laboratory evolution of DNA-binding activity and...

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Publicado no:Nat Methods
Main Authors: Hubbard, Basil P., Badran, Ahmed H., Zuris, John A., Guilinger, John P., Davis, Kevin M., Chen, Liwei, Tsai, Shengdar Q., Sander, Jeffry D., Joung, J. Keith, Liu, David R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4589463/
https://ncbi.nlm.nih.gov/pubmed/26258293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3515
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