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A diphtheria toxin resistance marker for in vitro and in vivo selection of stably transduced human cells
We developed a selectable marker rendering human cells resistant to Diphtheria Toxin (DT). The marker (DT(R)) consists of a primary microRNA sequence engineered to downregulate the ubiquitous DPH2 gene, a key enzyme for the biosynthesis of the DT target diphthamide. DT(R) expression in human cells i...
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| Publicado no: | Sci Rep |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4588510/ https://ncbi.nlm.nih.gov/pubmed/26420058 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep14721 |
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