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Analysis of Drug-Protein Binding using On-Line Immunoextraction and High-Performance Affinity Microcolumns: Studies with Normal and Glycated Human Serum Albumin

A method combining on-line immunoextraction microcolumns with high-performance affinity chromatography (HPAC) was developed and tested for use in examining drug-protein interactions with normal or modified proteins. Normal human serum albumin (HSA) and glycated HSA were used as model proteins for th...

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Detalhes bibliográficos
Publicado no:J Chromatogr A
Main Authors: Matsuda, Ryan, Jobe, Donald, Beyersdorf, Jared, Hage, David S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4587353/
https://ncbi.nlm.nih.gov/pubmed/26381571
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chroma.2015.09.023
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