Initiation of heteroduplex-loop repair by T4-encoded endonuclease VII in vitro.
Heteroduplex DNAs with single-stranded loops of 51 nt or 8 nt were constructed in vitro and used in reactions with purified endonuclease VII (endo VII) from phage T4. The enzyme makes double-strand breaks by introducing pairs of staggered nicks flanking the loops. Regardless of loop-size the nicking...
Guardado en:
| Publicado en: | EMBO J |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Nature Publishing Group
1988
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC458405/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3409872/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1988.tb02972.x |
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