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Initiation of heteroduplex-loop repair by T4-encoded endonuclease VII in vitro.

Heteroduplex DNAs with single-stranded loops of 51 nt or 8 nt were constructed in vitro and used in reactions with purified endonuclease VII (endo VII) from phage T4. The enzyme makes double-strand breaks by introducing pairs of staggered nicks flanking the loops. Regardless of loop-size the nicking...

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Udgivet i:EMBO J
Principais autores: Kleff, S, Kemper, B
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 1988
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC458405/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3409872/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1988.tb02972.x
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