Engineering Streptavidin and a Streptavidin-Binding Peptide with Infinite Binding Affinity and Reversible Binding Capability: Purification of a Tagged Recombinant Protein to High Purity via Affinity-Driven Thiol Coupling

To extend and improve the utility of the streptavidin-binding peptide tag (SBP-tag) in applications ranging from affinity purification to the reversible immobilization of recombinant proteins, a cysteine residue was introduced to the streptavidin mutein SAVSBPM18 and the SBP-tag to generate SAVSBPM3...

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Detalles Bibliográficos
Publicado en:PLoS One
Main Authors: Fogen, Dawson, Wu, Sau-Ching, Ng, Kenneth Kai-Sing, Wong, Sui-Lam
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science 2015
Assuntos:
Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4583386/
https://ncbi.nlm.nih.gov/pubmed/26406477
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0139137
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