DNA-segment-facilitated dissociation of Fis and NHP6A from DNA detected via single-molecule mechanical response

The rate of dissociation of a DNA-protein complex is often considered to be a property of that complex, without dependence on other nearby molecules in solution. We study the kinetics of dissociation of the abundant E. coli nucleoid protein Fis from DNA, using a single-molecule mechanics assay. The...

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Veröffentlicht in:J Mol Biol
Hauptverfasser: Giuntoli, Rebecca D., Linzer, Nora B., Banigan, Edward J., Sing, Charles E., de la Cruz, Monica Olvera, Graham, John S., Johnson, Reid C., Marko, John F.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2015
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4579036/
https://ncbi.nlm.nih.gov/pubmed/26220077
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2015.07.015
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