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Thermodynamic Protein Destabilization by GFP Tagging: A Case of Interdomain Allostery
The Engrailed Homeodomain (EnHD) transcription factor of Drosophila melanogaster was fused to the enhanced green fluorescent protein (eGFP) either at its C- or N-terminus via three- or ten-residue flexible linkers. Here, we show that EnHD undergoes destabilization upon fusing it to eGFP regardless o...
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| Опубликовано в: : | Biophys J |
|---|---|
| Главные авторы: | , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
The Biophysical Society
2015
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4576145/ https://ncbi.nlm.nih.gov/pubmed/25998254 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2015.04.032 |
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