Thermodynamic Protein Destabilization by GFP Tagging: A Case of Interdomain Allostery

The Engrailed Homeodomain (EnHD) transcription factor of Drosophila melanogaster was fused to the enhanced green fluorescent protein (eGFP) either at its C- or N-terminus via three- or ten-residue flexible linkers. Here, we show that EnHD undergoes destabilization upon fusing it to eGFP regardless o...

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Bibliographic Details
Published in:Biophys J
Main Authors: Sokolovski, Miri, Bhattacherjee, Arnab, Kessler, Naama, Levy, Yaakov, Horovitz, Amnon
Format: Artigo
Language:Inglês
Published: The Biophysical Society 2015
Subjects:
Proteins and Nucleic Acids
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4576145/
https://ncbi.nlm.nih.gov/pubmed/25998254
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2015.04.032
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