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Monitoring the heat-induced structural changes of alkaline phosphatase by molecular modeling, fluorescence spectroscopy and inactivation kinetics investigations

The heat induced conformational changes of calf alkaline phosphatase (ALP) were analyzed using different methods, based on fluorescence spectroscopy, molecular modeling and inactivation studies. Experimental studies were conducted in buffer solution in the temperature range between 25 and 70 °C. Mol...

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Bibliografiske detaljer
Udgivet i:J Food Sci Technol
Main Authors: Dumitrașcu, Loredana, Stănciuc, Nicoleta, Aprodu, Iuliana, Ciuciu, Ana-Maria, Alexe, Petru, Bahrim, Gabriela Elena
Format: Artigo
Sprog:Inglês
Udgivet: Springer India 2015
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4573144/
https://ncbi.nlm.nih.gov/pubmed/26396374
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13197-015-1719-1
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