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Monitoring the heat-induced structural changes of alkaline phosphatase by molecular modeling, fluorescence spectroscopy and inactivation kinetics investigations

The heat induced conformational changes of calf alkaline phosphatase (ALP) were analyzed using different methods, based on fluorescence spectroscopy, molecular modeling and inactivation studies. Experimental studies were conducted in buffer solution in the temperature range between 25 and 70 °C. Mol...

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Bibliografske podrobnosti
izdano v:J Food Sci Technol
Main Authors: Dumitrașcu, Loredana, Stănciuc, Nicoleta, Aprodu, Iuliana, Ciuciu, Ana-Maria, Alexe, Petru, Bahrim, Gabriela Elena
Format: Artigo
Jezik:Inglês
Izdano: Springer India 2015
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4573144/
https://ncbi.nlm.nih.gov/pubmed/26396374
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13197-015-1719-1
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