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Prokaryotic expression and renaturation of engineering chimeric Fab antibody against human hepatoma
AIM: To express chimeric Fd (cFd) and chimeric light chain (cL) in E. coli respectively and refold them into chimeric Fab (cFab) antibody. METHODS: cFd and cL genes were respectively inserted into the prokaryotic expression vector pET32a to construct recombinant vectors pET32a/cFd and pET32a/cL. The...
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| Publicado no: | World J Gastroenterol |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Baishideng Publishing Group Inc
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4572327/ https://ncbi.nlm.nih.gov/pubmed/15237428 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v10.i14.2029 |
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