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Prokaryotic expression and renaturation of engineering chimeric Fab antibody against human hepatoma

AIM: To express chimeric Fd (cFd) and chimeric light chain (cL) in E. coli respectively and refold them into chimeric Fab (cFab) antibody. METHODS: cFd and cL genes were respectively inserted into the prokaryotic expression vector pET32a to construct recombinant vectors pET32a/cFd and pET32a/cL. The...

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Vydáno v:World J Gastroenterol
Hlavní autoři: Xing, Jin-Liang, Yang, Xiang-Min, Yao, Xi-Ying, Song, Fei, Chen, Zhi-Nan
Médium: Artigo
Jazyk:Inglês
Vydáno: Baishideng Publishing Group Inc 2004
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4572327/
https://ncbi.nlm.nih.gov/pubmed/15237428
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v10.i14.2029
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