Temperature Effects on Kinetic Parameters and Substrate Affinity of Cel7A Cellobiohydrolases

We measured hydrolytic rates of four purified cellulases in small increments of temperature (10–50 °C) and substrate loads (0–100 g/liter) and analyzed the data by a steady state kinetic model that accounts for the processive mechanism. We used wild type cellobiohydrolases (Cel7A) from mesophilic Hy...

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Bibliografiske detaljer
Udgivet i:J Biol Chem
Main Authors: Sørensen, Trine Holst, Cruys-Bagger, Nicolaj, Windahl, Michael Skovbo, Badino, Silke Flindt, Borch, Kim, Westh, Peter
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Biochemistry and Molecular Biology 2015
Fag:
Enzymology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4571970/
https://ncbi.nlm.nih.gov/pubmed/26183777
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M115.658930
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