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Pre-steady-state Kinetics for Hydrolysis of Insoluble Cellulose by Cellobiohydrolase Cel7A

The transient kinetic behavior of enzyme reactions prior to the establishment of steady state is a major source of mechanistic information, yet this approach has not been utilized for cellulases acting on their natural substrate, insoluble cellulose. Here, we elucidate the pre-steady-state regime fo...

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Detalhes bibliográficos
Main Authors: Cruys-Bagger, Nicolaj, Elmerdahl, Jens, Praestgaard, Eigil, Tatsumi, Hirosuke, Spodsberg, Nikolaj, Borch, Kim, Westh, Peter
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3365755/
https://ncbi.nlm.nih.gov/pubmed/22493488
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.334946
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