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Substrate binding in the processive cellulase Cel7A: Transition state of complexation and roles of conserved tryptophan residues
Cellobiohydrolases effectively degrade cellulose and are of biotechnological interest because they can convert lignocellulosic biomass to fermentable sugars. Here, we implemented a fluorescence-based method for real-time measurements of complexation and decomplexation of the processive cellulase Cel...
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| Pubblicato in: | J Biol Chem |
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| Autori principali: | , , , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
American Society for Biochemistry and Molecular Biology
2020
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7008363/ https://ncbi.nlm.nih.gov/pubmed/31848226 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA119.011420 |
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