Substrate binding in the processive cellulase Cel7A: Transition state of complexation and roles of conserved tryptophan residues

Cellobiohydrolases effectively degrade cellulose and are of biotechnological interest because they can convert lignocellulosic biomass to fermentable sugars. Here, we implemented a fluorescence-based method for real-time measurements of complexation and decomplexation of the processive cellulase Cel...

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Dades bibliogràfiques
Publicat a:J Biol Chem
Autors principals: Røjel, Nanna, Kari, Jeppe, Sørensen, Trine Holst, Badino, Silke F., Morth, J. Preben, Schaller, Kay, Cavaleiro, Ana Mafalda, Borch, Kim, Westh, Peter
Format: Artigo
Idioma:Inglês
Publicat: American Society for Biochemistry and Molecular Biology 2020
Matèries:
Enzymology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7008363/
https://ncbi.nlm.nih.gov/pubmed/31848226
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA119.011420
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