Substrate binding in the processive cellulase Cel7A: Transition state of complexation and roles of conserved tryptophan residues

Cellobiohydrolases effectively degrade cellulose and are of biotechnological interest because they can convert lignocellulosic biomass to fermentable sugars. Here, we implemented a fluorescence-based method for real-time measurements of complexation and decomplexation of the processive cellulase Cel...

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Bibliografiske detaljer
Udgivet i:J Biol Chem
Main Authors: Røjel, Nanna, Kari, Jeppe, Sørensen, Trine Holst, Badino, Silke F., Morth, J. Preben, Schaller, Kay, Cavaleiro, Ana Mafalda, Borch, Kim, Westh, Peter
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Biochemistry and Molecular Biology 2020
Fag:
Enzymology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7008363/
https://ncbi.nlm.nih.gov/pubmed/31848226
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA119.011420
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