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Substrate binding in the processive cellulase Cel7A: Transition state of complexation and roles of conserved tryptophan residues

Cellobiohydrolases effectively degrade cellulose and are of biotechnological interest because they can convert lignocellulosic biomass to fermentable sugars. Here, we implemented a fluorescence-based method for real-time measurements of complexation and decomplexation of the processive cellulase Cel...

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Detalles Bibliográficos
Publicado en:J Biol Chem
Autores principales: Røjel, Nanna, Kari, Jeppe, Sørensen, Trine Holst, Badino, Silke F., Morth, J. Preben, Schaller, Kay, Cavaleiro, Ana Mafalda, Borch, Kim, Westh, Peter
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Biochemistry and Molecular Biology 2020
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7008363/
https://ncbi.nlm.nih.gov/pubmed/31848226
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA119.011420
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