Substrate binding in the processive cellulase Cel7A: Transition state of complexation and roles of conserved tryptophan residues

Cellobiohydrolases effectively degrade cellulose and are of biotechnological interest because they can convert lignocellulosic biomass to fermentable sugars. Here, we implemented a fluorescence-based method for real-time measurements of complexation and decomplexation of the processive cellulase Cel...

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Salvato in:
Dettagli Bibliografici
Pubblicato in:J Biol Chem
Autori principali: Røjel, Nanna, Kari, Jeppe, Sørensen, Trine Holst, Badino, Silke F., Morth, J. Preben, Schaller, Kay, Cavaleiro, Ana Mafalda, Borch, Kim, Westh, Peter
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Biochemistry and Molecular Biology 2020
Soggetti:
Enzymology
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7008363/
https://ncbi.nlm.nih.gov/pubmed/31848226
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA119.011420
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