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RNA polymerase idling and clearance in gal promoters: use of supercoiled minicircle DNA template made in vivo.

We have developed an in vivo system to engender supercoiled "minicircle" DNA containing a single promoter by using the integrative recombination system of bacteriophage lambda. The resulting minicircle templates allow quantitative analysis of the stages of transcription initiation from a promoter, i...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Choy, H E, Adhya, S
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1993
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC45685/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8380640/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.2.472
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