RNA polymerase idling and clearance in gal promoters: use of supercoiled minicircle DNA template made in vivo.
We have developed an in vivo system to engender supercoiled "minicircle" DNA containing a single promoter by using the integrative recombination system of bacteriophage lambda. The resulting minicircle templates allow quantitative analysis of the stages of transcription initiation from a promoter, i...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1993
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC45685/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8380640/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.2.472 |
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