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Genome-Wide Fitness and Genetic Interactions Determined by Tn-seq, a High-Throughput Massively Parallel Sequencing Method for Microorganisms

The lagging annotation of bacterial genomes and the inherent genetic complexity of many phenotypes is hindering the discovery of new drug targets and the development of new antimicrobials and vaccines. Here we present the method Tn-seq, with which it has become possible to quantitatively determine f...

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Dettagli Bibliografici
Pubblicato in:Curr Protoc Mol Biol
Autori principali: van Opijnen, Tim, Lazinski, David W., Camilli, Andrew
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4568079/
https://ncbi.nlm.nih.gov/pubmed/24733243
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471142727.mb0716s106
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