A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation

Biochemical investigation of protein phosphorylation events is limited by inefficient production of the phosphorylated and non-phosphorylated forms of full-length proteins. Here using a genomically recoded strain of E. coli with a flexible UAG codon we produce site-specific serine- or phosphoserine-...

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Xehetasun bibliografikoak
Argitaratua izan da:Nat Commun
Egile Nagusiak: Pirman, Natasha L., Barber, Karl W., Aerni, Hans R., Ma, Natalie J., Haimovich, Adrian D., Rogulina, Svetlana, Isaacs, Farren J., Rinehart, Jesse
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Nature Pub. Group 2015
Gaiak:
Article
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC4566969/
https://ncbi.nlm.nih.gov/pubmed/26350500
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms9130
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