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Robust production of recombinant phosphoproteins using cell-free protein synthesis
Understanding the functional and structural consequences of site-specific protein phosphorylation has remained limited by our inability to produce phosphoproteins at high yields. Here we address this limitation by developing a cell-free protein synthesis (CFPS) platform that employs crude extracts f...
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| Publicado no: | Nat Commun |
|---|---|
| Main Authors: | , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Pub. Group
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4566161/ https://ncbi.nlm.nih.gov/pubmed/26350765 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms9168 |
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