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A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation
Biochemical investigation of protein phosphorylation events is limited by inefficient production of the phosphorylated and non-phosphorylated forms of full-length proteins. Here using a genomically recoded strain of E. coli with a flexible UAG codon we produce site-specific serine- or phosphoserine-...
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| Publicado no: | Nat Commun |
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| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Pub. Group
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4566969/ https://ncbi.nlm.nih.gov/pubmed/26350500 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms9130 |
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