Robust production of recombinant phosphoproteins using cell-free protein synthesis

Understanding the functional and structural consequences of site-specific protein phosphorylation has remained limited by our inability to produce phosphoproteins at high yields. Here we address this limitation by developing a cell-free protein synthesis (CFPS) platform that employs crude extracts f...

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Vydáno v:Nat Commun
Hlavní autoři: Oza, Javin P., Aerni, Hans R., Pirman, Natasha L., Barber, Karl W., ter Haar, Charlotte M., Rogulina, Svetlana, Amrofell, Matthew B., Isaacs, Farren J., Rinehart, Jesse, Jewett, Michael C.
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Pub. Group 2015
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4566161/
https://ncbi.nlm.nih.gov/pubmed/26350765
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms9168
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