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Interplay of catalytic subsite residues in the positioning of α-d-glucose 1-phosphate in sucrose phosphorylase

Kinetic and molecular docking studies were performed to characterize the binding of α-d-glucose 1-phosphate (αGlc 1-P) at the catalytic subsite of a family GH-13 sucrose phosphorylase (from L. mesenteroides) in wild-type and mutated form. The best-fit binding mode of αGlc 1-P dianion had the phospha...

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Detalhes bibliográficos
Publicado no:Biochem Biophys Rep
Main Authors: Wildberger, Patricia, Aish, Gaia A., Jakeman, David L., Brecker, Lothar, Nidetzky, Bernd
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4554294/
https://ncbi.nlm.nih.gov/pubmed/26380381
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrep.2015.04.001
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