Uncovering the Stoichiometry of Pyrococcus furiosus RNase P, a Multi-subunit Catalytic Ribonucleoprotein Complex, by Surface-Induced Dissociation and Ion Mobility-Mass Spectrometry

We demonstrate that surface-induced dissociation (SID) coupled with ion mobility-mass spectrometry (IM-MS) is a powerful tool for determining the stoichiometry and quaternary structure of a multi-subunit ribonucleoprotein (RNP) complex assembled in Mg(2+). We investigated here Pyrococcus furiosus (P...

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Detalhes bibliográficos
Publicado no:Angew Chem Int Ed Engl
Main Authors: Ma, Xin, Lai, Lien B., Lai, Stella M., Tanimoto, Akiko, Foster, Mark P., Wysocki, Vicki H., Gopalan, Venkat
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4552314/
https://ncbi.nlm.nih.gov/pubmed/25195671
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201405362
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