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Polar Fixation of Plasmids during Recombinant Protein Production in Bacillus megaterium Results in Population Heterogeneity

During the past 2 decades, Bacillus megaterium has been systematically developed for the gram-per-liter scale production of recombinant proteins. The plasmid-based expression systems employed use a xylose-controlled promoter. Protein production analyses at the single-cell level using green fluoresce...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Münch, Karin M., Müller, Johannes, Wienecke, Sarah, Bergmann, Simone, Heyber, Steffi, Biedendieck, Rebekka, Münch, Richard, Jahn, Dieter
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2015
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4551236/
https://ncbi.nlm.nih.gov/pubmed/26116677
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00807-15
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